Part:BBa_K2623013:Design

Kai's phosphorylation core oscllator builder and SfYFP reportor for pKaiBC

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 627
  Illegal AgeI site found at 1442
  Illegal AgeI site found at 2153
  Illegal AgeI site found at 2162
  Illegal AgeI site found at 2168
  Illegal AgeI site found at 2492
  Illegal AgeI site found at 2519
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We used T4 DNA ligase to construct the circuit.

Source

This part contains the coding region of the Prochlorococcus (Synechococcus) elongatus PCC7942 gene.